PLANTS HAVING INCREASED TOLERANCE TO HERBICIDES

摘要

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type hydroxyphenyl pyruvate dioxygenase or a mutated hydroxyphenyl pyruvate dioxygenase (mut-HPPD) which is resistant or tolerant to a HPPD-inhibiting herbicide and/or a nucleotide sequence encoding a wild-type homogentisate solanesyl transferase or a mutated homogentisate solanesyl transferase (mut-HST) which is resistant or tolerant to a HPPD-inhibiting herbicide, applying to said site an effective amount of said herbicide. The invention further refers to plants comprising mut-HPPD, and methods of obtaining such plants.

主权项

1. A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
a) providing, at said site, a plant that comprises at least one nucleic acid comprising
(i) a nucleotide sequence encoding a wild-type hydroxyphenyl pyruvate dioxygenase or a mutated hydroxyphenyl pyruvate dioxygenase (mut-HPPD) which is resistant or tolerant to a HPPD-inhibiting herbicide and/or(ii) a nucleotide sequence encoding a wild-type homogentisate solanesyl transferase or a mutated homogentisate solanesyl transferase (mut-HST) which is resistant or tolerant to a HPPD-inhibiting herbicide b) applying to said site an effective amount of said herbicide. wherein the nucleotide sequence of (i) comprises the sequence of SEQ ID NO: 1, 51, 3, 4, 6, 7, 9, 10, 12, 13, 15, 16, 18, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 52, 54, 56, 68, 69, or a variant or derivative thereof, and wherein the HPPD inhibiting herbicide is a benzamide, and wherein the mut-HPPD comprises a polypeptide which differs from an amino acid sequence of a wildtype HPPD at one or more positions corresponding to the following positions of SEQ ID NO:2:
the amino acid corresponding to or at position 236 is other than alanine;the amino acid corresponding to or at position 411 is other than glutamic acid;the amino acid corresponding to or at position 320 is other than leucine;the amino acid corresponding to or at position 403 is other than glycine;the amino acid corresponding to or at position 334 is other than leucine;the amino acid corresponding to or at position 353 is other than leucine;the amino acid corresponding to or at position 321 is other than proline;the amino acid corresponding to or at position 212 is other than valine;the amino acid corresponding to or at position 407 is other than glycine;the amino acid corresponding to or at position 377 is other than phenylalanine;the amino acid corresponding to or at position 412 is other than leucine;the amino acid corresponding to or at position 278 is other than glutamine;the amino acid corresponding to or at position 406 is other than lysine;the amino acid corresponding to or at position 404 is other than phenylalanine;the amino acid corresponding to or at position 409 is other than phenylalanine;the amino acid corresponding to or at position 416 is other than isoleucine;the amino acid corresponding to or at position 250 is other than leucine;the amino acid corresponding to or at position 267 is other than asparagine;the amino acid corresponding to or at position 252 is other than serine;the amino acid corresponding to or at position 265 is other than proline;the amino acid corresponding to or at position 371 is other than glycine;the amino acid corresponding to or at position 375 is other than threonine;the amino acid corresponding to or at position 309 is other than arginine;the amino acid corresponding to or at position 279 is other than isoleucine;the amino acid corresponding to or at position 366 is other than phenylalanine;the amino acid corresponding to or at position 238 is other than phenylalanine;the amino acid corresponding to or at position 213 is other than valine;the amino acid corresponding to or at position 215 is other than asparagine;the amino acid corresponding to or at position 410 is other than serine;the amino acid corresponding to or at position 254 is other than valine.