发明名称 |
METHODS AND MEANS FOR ENHANCING RNA PRODUCTION |
摘要 |
The present invention relates to a method for synthesizing an RNA molecule of a given sequence, comprising the step of determining the fraction (1) for each of the four nucleotides G, A, C and U in said RNA molecule, and the step of synthesizing said RNA molecule by in vitro transcription in a sequence-optimized reaction mix, wherein said sequence-optimized reaction mix comprises the four ribonucleoside triphosphates GTP, ATP, CTP and UTP, wherein the fraction (2) of each of the four ribonucleoside triphosphates in the sequence-optimized reaction mix corresponds to the fraction (1) of the respective nucleotide in said RNA molecule, a buffer, a DNA template, and an RNA polymerase. Further, the present invention relates to a bioreactor (1) for synthesizing RNA molecules of a given sequence, the bioreactor (1) having a reaction module (2) for carrying out in vitro RNA transcription reactions in a sequence-optimized reaction mix, a capture module (3) for temporarily capturing the transcribed RNA molecules, and a control module (4) for controlling the infeed of components of the sequence-optimized reaction mix into the reaction module (2), wherein the reaction module (2) comprises a filtration membrane (21) for separating nucleotides from the reaction mix, and the control of the infeed of components of the sequence-optimized reaction mix by the control module (4) is based on a measured concentration of separated nucleotides. |
申请公布号 |
US2017114378(A1) |
申请公布日期 |
2017.04.27 |
申请号 |
US201515317579 |
申请日期 |
2015.06.10 |
申请人 |
CUREVAC AG |
发明人 |
WOCHNER Aniela;ROOS Tilmann;KETTERER Thomas |
分类号 |
C12P19/34;C12M1/34;C12M1/36;C12M1/00;C12M1/40;C12M3/00 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for synthesizing an RNA molecule of a given sequence, comprising the following steps:
a) determining the fraction (1) for each of the four nucleotides G, A, C and U in said RNA molecule, and b) synthesizing said RNA molecule by in vitro transcription in a sequence-optimized reaction mix, wherein said sequence-optimized reaction mix comprises the four ribonucleoside triphosphates (NTPs) GTP, ATP, CTP and UTP, wherein the fraction (2) of each of the four ribonucleoside triphosphates in the sequence-optimized reaction mix corresponds to the fraction (1) of the respective nucleotide in said RNA molecule, a buffer, a DNA template, and an RNA polymerase. |
地址 |
Tübingen DE |