Auto-recognizing therapeutic RNA/DNA chimeric nanoparticles (NP)

摘要

Auto-recognizing therapeutic R/DNA chimeric nanoparticles (R/DNA NP) are described that are pairs of DNA/RNA hybrids where the DNA molecules have complementary toehold sequences that promote the re-association of the R/DNA NPs when mixed resulting in the formation of RNA/RNA duplexes that act as siRNAs.

主权项

1. An R/DNA chimeric polyfunctional nanoparticle (R/DNA NP) comprising at least two complementary auto-recognizing chimeric nanoparticles,
said first chimeric nanoparticle comprising a first DNA oligonucleotide that is hybridized along its length to one or more complementary first RNA oligonucleotides, said second chimeric nanoparticle comprising a second DNA oligonucleotide that is hybridized along its length to one or more complementary second RNA oligonucleotides, wherein the first DNA oligonucleotide is complementary to the second DNA oligonucleotide and the first RNA oligonucleotides are complementary to the second RNA oligonucleotides, wherein the first DNA oligonucleotide comprises a 5′ toehold sequence and the second DNA oligonucleotide comprises a 3′ toehold sequence that is complementary to the 5′ toehold sequence, and wherein a DNA duplex and one or more functional RNA duplexes are formed upon the hybridization of the 5′ and 3′ toeholds and the subsequent reassociation of the complementary first and second RNA oligonucleotides.