| 发明名称 | Compositions and methods for peptide expression and purification using a type III secretion system | ||
| 摘要 | Disclosed are compositions and methods for expressing and purifying a peptide of interest using a Flagellar Type III secretion system. Disclosed are nucleic acid sequences that contain a FlgM nucleic acid sequence, a cleavage site, and a nucleic acid sequence of interest. Also disclosed are polypeptides that contain FlgM, a cleavage site and a peptide of interest. Methods of producing polypeptides that have FlgM, a cleavage site and a peptide of interest are provided. | ||
| 申请公布号 | US9630997(B2) | 申请公布日期 | 2017.04.25 |
| 申请号 | US201314404919 | 申请日期 | 2013.05.30 |
| 申请人 | University of Utah Research Foundation | 发明人 | Hughes Kelly T.;Olivera Baldomero M. |
| 分类号 | C07K14/245;C12N15/74;C07K14/255;C07K14/34;C07K14/435;C07K14/46;C12P21/02;C12N15/62;C12N15/70 | 主分类号 | C07K14/245 |
| 代理机构 | Sterne, Kessler, Goldstein & Fox P.L.L.C. | 代理人 | Sterne, Kessler, Goldstein & Fox P.L.L.C. |
| 主权项 | 1. A vector comprising a FlgM nucleic acid sequence operably linked to a nucleic acid sequence encoding a purification tag, a cleavage site, a nucleic acid sequence of interest, and a transcription control element (TCE), wherein the TCE is heterologous to the FlgM nucleic acid sequence and wherein the 5′ to 3′ order of: the sequences is the FlgM nucleic acid sequence, the nucleic acid sequence encoding a purification tag, the cleavage site, and the nucleic acid sequence of interest. | ||
| 地址 | Salt Lake City UT US | ||