Exosome transfer of nucleic acids to cells

摘要

Methods for introducing nucleic acids to cells via exosomes for use in gene modulation and therapy, such as for gene silencing and to introduce genetic material into cells to compensate for abnormal genes or to induce or repress a process in the recipient cell.

主权项

1. A method of transferring genetic material to recipient cells, the method comprising:
a) isolating exosomes produced and released by a donor mast cell, wherein the isolating is performed in vitro and the isolated exosomes lack genetic material; b) transforming, transfecting, or microinjecting selected genetic material to the isolated exosomes in vitro to produce modified exosomes; and c) administering the modified exosomes to recipient cells thereby transferring the selected genetic material from the modified exosomes to the cytoplasm of the recipient cells.