Microscopy imaging device with advanced imaging properties

摘要

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 μm resolution for an image of the field of view.

主权项

1. An epifluorescence microscopy system, the system comprising:
an optical excitation arrangement configured to direct light over an area encompassed within a field of view containing an imaging target along a specimen plane; an imaging circuit including an optical sensor array configured to generate image data from fluorescence caused by an interaction between the directed light and the imaging target; a synchronization circuit in communication with the imaging circuit and configured to interface with an external optical-data processing system that provides visual feedback of the image data; and an optical arrangement configured to direct the fluorescence through a dichroic mirror to the optical sensor array with sufficient intensity and focus for the image data to reach cellular level brain imaging, wherein the optical arrangement comprises an objective lens configured to direct the fluorescence to the optical sensor array, wherein the epifluorescence microscopy system is configured to be attached and reattached to a base plate of a supportive structure for allowing precise alignment of the epifluorescence microscopy system for repeated imaging of the field of view containing the imaging target during chronic experiments, and wherein the epifluorescence microscopy system has a dimension that does not exceed 1 inch along a plane parallel to the specimen plane.