Administration and monitoring of nitric oxide in ex vivo fluids

摘要

Described are systems and methods for monitoring administration of nitric oxide (NO) to ex vivo fluids. Examples of such fluids include blood in extracorporeal membrane oxygenation (ECMO) circuits or perfusion fluids used for preserving ex vivo organs prior to transplanting in a recipient. The systems and methods described herein provide for administering nitric oxide to the fluid, monitoring nitric oxide or a nitric oxide marker in the fluid, and adjusting the nitric oxide administration.

主权项

1. A method of monitoring nitric oxide administration comprising:
administering nitric oxide to an ex vivo fluid comprising red blood cells by contacting the ex vivo fluid with a gas comprising a nitric oxide concentration in the range from 1 ppm to 300 ppm; monitoring methemoglobin and at least one of nitric oxide or NOx in the ex vivo fluid after administrating nitric oxide to the ex vivo fluid; adjusting the nitric oxide administration based on the monitoring of the methemoglobin and the nitric oxide or NOx; and contacting cells with the ex vivo fluid after administering nitric oxide to the ex vivo fluid.