| 摘要 |
A protein conferring resistance to Fusarium Head Blight disease (FHB) is described, along with the DNA sequence of the corresponding gene and mRNA copy (cDNA). The cDNA of Fhb1 gene can be used to produce genetically-modified plants having increased resistance to FHB, particularly in wheat, barley and other plants affected by the disease. The protein has antifungal properties and inhibits fungal growth, thereby providing a means for reducing DON toxin in grains. Conserved functional domains are identified in the protein. Genetically-modified plants having increased resistance FHB are also described, along with methods for producing such genetically-modified plants. |
| 主权项 |
1. A genetically-modified plant cell having increased resistance to FHB disease relative to a control plant cell, said genetically-modified plant cell comprising an exogenous Fhb1 nucleic acid encoding a protein, or conserved domain or residue thereof, which confers resistance to FHB, wherein said exogenous nucleic acid:
(a) comprises a nucleotide sequence of SEQ ID NO: 1, 2, 4, 5 or 6; (b) comprises a nucleotide sequence having at least about 70% sequence identity to SEQ ID NO:1, 2, 4, 5 or 6; (c) encodes a protein comprising the amino acid sequence of SEQ ID NO:3, 7, 8, or 9; or (d) encodes a protein having at least about 70% amino acid identity with SEQ ID NO:3, 7, 8, or 9 and retaining the functional characteristics of the protein having the amino acid sequence of SEQ ID NO:3, 7, 8, or 9, wherein said functional characteristics comprise the ability to confer resistance to FHB. |
