HIGH-RESOLUTION THREE-DIMENSIONAL IMAGING OF MAMMALIAN HEARTS

摘要

Methods and materials for high-resolution three-dimensional imaging of mammalian tissues, such as heart and intestine, are described. Both methods of tissue preparation for imaging and methods of imaging are described, as well as kits comprising materials and media for use in the methods.

主权项

1. A method of preparing a tissue sample for high-resolution three-dimensional tissue imaging, comprising:
(a) embedding a fixed tissue sample in an embedding medium; (b) sectioning the embedded tissue into sections of 20-1000 μm thickness; (c) staining the sectioned tissue; (d) mounting the stained 20-1000 μm thick tissue sections on a coverslip and glass slide; and (e) clearing the 20-1000 μm thick tissue sections with a reagent that solubilizes lipids, increases sample transparency, and increases sample refractive index.