METHOD FOR DETERMINATION OF A PROTEIN

摘要

The present invention concerns a method for determining the concentration of a protein in a gastrointestinal (GI) tract sample taken from a human or an animal. The present invention is characterized by the feature that a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10,000 is obtained. The present invention leads to a significant improvement of the technical situation, and provides a simple, sensitive and specific determination tool of proteins in GI tract samples. The determination of proteins, e.g. calprotectin, elastases or hemoglobin, in GI tract samples leads to more accurate and reproducible results.

主权项

1. A method for determining the concentration of a protein in a gastrointestinal (GI) tract sample taken from a human or an animal, comprising the steps of
a) Collecting said sample and b) Mixing the sample of step a) with a determined amount of a buffered aqueous extraction medium; c) Homogenizing the mixture of step b) and d) Performing an immunoassay by using the mixture obtained in step c); e) Determining the concentration of the protein, wherein in step b) a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10000 is obtained.