PROGENITOR CELLS AND METHODS FOR PREPARING AND USING THE SAME

摘要

Provided are methods for isolating subpopulations of stem cells. In some embodiments, the presently disclosed methods include selecting subsets of cells that are positive for CD34 or Sca-1, are further positive for one or more of FSHR, LHCGR, PRLR, AR, ESRα, ESRβ, and PGR; and are negative for each of CD45R/B220, Gr-1, TCRαβ, TCRγδ, CD11b, and Ter-119. In some embodiments, the subpopulations are further fractioning into CD45− and CD45+ fractions. Also provided are populations of stem cells isolated by the presently disclosed methods, compositions that include the presently disclosed subpopulations in pharmaceutically acceptable carriers, methods for expanding stem cells, methods for stimulating proliferation of MSCs, methods for treating subjects suffering from exposure to radiation, and methods for producing gametes in vitro.

主权项

1. A method for isolating a subpopulation of stem cells from a population of cells, the method comprising:
(a) providing a population of CD34+ human cells or Sca-1+ murine cells suspected of containing stem cells; and (b) selecting from the population of cells a subset of cells that are positive for a gene product selected from the group consisting of follicle-stimulating hormone receptor (FSHR), luteinizing hormone/choriogonadotropin receptor (LHCGR), prolactin receptor (PRLR), androgen receptor (AR), estrogen receptor α (ESRα), estrogen receptor β (ESRβ), and progesterone receptor (PGR); and are negative for each of CD45R/B220, Gr-1, TCRαβ, TCRγδ, CD11b, and Ter-119,whereby a subpopulation of stem cells is isolated.