NUCLEIC ACID AMPLIFICATION REAGENT, NUCLEIC ACID AMPLIFICATION CARTRIDGE, AND NUCLEIC ACID AMPLIFICATION METHOD

摘要

A nucleic acid amplification reagent includes a first probe which anneals to a target nucleic acid in a region sandwiched between a site to which the 3′ end of a forward primer anneals and a site to which the 3′ end of a reverse primer anneals in a template nucleic acid, and a second probe which anneals to a nucleic acid other than the target nucleic acid in the region. Each of the first probe and the second probe includes a dye which emits light in a wavelength band mutually overlapping partially.

主权项

1. A nucleic acid amplification reagent, which is used for amplifying a target nucleic acid in a template nucleic acid, comprising:
a first probe which anneals to the target nucleic acid in a region sandwiched between a site to which the 3′ end of a forward primer anneals and a site to which the 3′ end of a reverse primer anneals in the template nucleic acid; and a second probe which anneals to a nucleic acid other than the target nucleic acid in the region, wherein each of the first probe and the second probe includes a dye which emits light in a wavelength band mutually overlapping partially.