METHODS FOR PRODUCING MICROCOMPARTMENTS

摘要

To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.

主权项

1. A method for producing bacterial microcompartments in a host organism, said method comprising introducing into an organism an expression vector comprising a heterologous nucleotide sequence or a functional fragment thereof comprising (a) an operably linked promoter that drives expression in the organism; (b) a ribosomal binding site sequence that controls expression efficiency in the host organism, and (c) a bacterial microcompartment gene or functional variant thereof derived from a bacteria for producing a bacterial microcompartment.