Elisa for VEGF

摘要

The vascular endothelial growth factor (VEGF) activity in a patient's bloodstream or other biological sample can serve as a diagnostic and prognostic index for cancer, diabetes, heart conditions, and other pathologies. Antibody-sandwich ELISA methods and kits for VEGF as an antigen are provided to detect types of VEGF levels in biological samples from animal models and human patients and can be used as a diagnostic/prognostic index.

主权项

1. An immunoassay kit for selectively detecting VEGF110+ forms in a biological sample, the forms including VEGF121, the kit comprising:
(a) as capture reagent, a monoclonal antibody against human VEGF comprising the six complementarity-determining regions (CDRs) of monoclonal antibody 5C3, obtainable from hybridoma 5C3.1.1 deposited at the American Type Culture Collection (ATCC) under accession number PTA-7737, wherein the monoclonal antibody binds specifically to the residues greater than 110 of human VEGF and binds VEGF121; and (b) as detection reagent, a detectable antibody comprising the six CDRs of monoclonal antibody A4.6.1, obtainable from hybridoma A4.6.1 deposited at ATCC under accession number HB10709, wherein said detectable antibody binds to the KDR and/or FLT1 receptor binding domains of VEGF or that binds to an epitope in VEGF 1-110.