| 发明名称 | Artificial immune system: methods of use | ||
| 摘要 | The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system. | ||
| 申请公布号 | US9625444(B2) | 申请公布日期 | 2017.04.18 |
| 申请号 | US201414221018 | 申请日期 | 2014.03.20 |
| 申请人 | SANOFI PASTEUR VAXDESIGN CORPORATION;THE SCRIPPS RESEARCH INSTITUTE;MASSACHUSETTS INSTITUTE OF TECHNOLOGY;THE WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH;MOUNT SINAI SCHOOL OF MEDICINE OF NEW YORK UNIVERSITY | 发明人 | Warren William L;Fahlenkamp Heather;Higbee Russell;Kachurin Anatoly;Li Conan;Nguyen Mike;Parkhill Robert;Sanchez-Schmitz Guzman;Irvine Darrell J.;Randolph Gwendalyn J.;Hacohen Nir;Torbett Bruce |
| 分类号 | C12N5/00;C12N5/02;A61K38/00;G01N33/50;C12N5/071 | 主分类号 | C12N5/00 |
| 代理机构 | Wenderoth, Lind & Ponack, L.L.P. | 代理人 | Wenderoth, Lind & Ponack, L.L.P. |
| 主权项 | 1. A method for generating dendritic cells, comprising: (a) culturing endothelial cells on an upper surface of a porous membrane, wherein said membrane is housed in an upper chamber of a cell culture well that is suspended over, and is separable from, a lower chamber of the well; (b) applying peripheral blood mononuclear cells (PBMCs) to the endothelial cells on the porous membrane of (a); (c) removing the upper chamber housing the porous membrane and endothelial cells from the well about 48 hours after application of the PBMCs; and (d) isolating dendritic cells from the lower chamber of the well. | ||
| 地址 | Orlando FL US | ||