System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

摘要

The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.

主权项

1. A method for analyzing tissue comprising:
obtaining a tissue sample; exposing the tissue sample to one or more different exogenous fluorophores excitable in a range of about 300 nm to about 200 nm and having a useful emission band from about 350 nm to about 900 nm, and including one or more fluorescent dyes or fluorescently labeled molecular probes that accumulate in tissue or cellular components; exciting, with an ultraviolet (UV) light source, the one or more different exogenous fluorophores with a first wavelength of UV light between about 200 nm and about 290 nm; collecting with an optical system, emissions from each of the one or more different exogenous fluorophores at a second wavelength different from the first wavelength of UV light, being from about 350 nm to about 950 nm, and being generated in response to the first wavelength of UV light.