| 发明名称 | System and method for nucleic acid amplification | ||
| 摘要 | An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule. | ||
| 申请公布号 | US9624519(B2) | 申请公布日期 | 2017.04.18 |
| 申请号 | US201414466063 | 申请日期 | 2014.08.22 |
| 申请人 | 454 Life Sciences Corporation | 发明人 | Godwin Brian Christopher;Shanbhag Priya;Mealmaker Craig Elder;Ferreri Gianni Calogero;Palmer Melinda;Wang Shally Hsueh-Wen |
| 分类号 | C12Q1/68;C12P19/34 | 主分类号 | C12Q1/68 |
| 代理机构 | Cooley LLP | 代理人 | Cooley LLP ;Elrifi Ivor R.;Stock Christina K. |
| 主权项 | 1. A method for generating a population of amplified concatamer products, comprising: amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a solid phase substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the solid phase substrate; amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to a sequence of an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule. | ||
| 地址 | Branford CT US | ||