| 主权项 |
1. A method for producing a genetically modified Myceliophthora deficient in at least one protease native to said Myceliophthora, comprising providing a Myceliophthora having protease activity, wherein said protease activity comprises lysing Myceliophthora cellobiohydrolase enzymes and said protease comprises an amino acid sequence having at least 98% identity with the polypeptide sequence set forth in SEQ ID NO:9; and mutating said Myceliophthora under conditions such that said protease is mutated to produce a protease-deficient Myceliophthora. |
