Tissue-specific differentiation matrices and uses thereof

摘要

In some aspects, this invention provides a method of making a bone marrow-derived tissue-specific stem cell proliferation, expansion, isolation and rejuvenation extracellular matrix. In other aspects, this invention provides a method of making a tissue-specific fibroblast-derived stem cell differentiation extracellular matrix. Also provided are methods of using such a cell-derived preservation or differentiation matrices to induce tissue-specific differentiation of pluripotent cells, repair damaged tissue, and treat a subject having a physiologic deficiency using the same.

主权项

1. A tissue-specific differentiation system comprising
a differentiation-inducing extracellular matrix generated ex vivo on a surface by culturing fibroblast cells on said surface, wherein the differentiation-inducing extracellular matrix is free of the fibroblast cells; and differentiated cells in contact with the differentiation-inducing extracellular matrix, wherein the differentiated cells are derived from isolated stem cells seeded onto the differentiation-inducing extracellular matrix and induced to undergo differentiation into differentiated cells of the same tissue type as the fibroblast cells b cultured on the differentiation-inducing extracellular extracellular matrix; wherein the fibroblast cells and differentiated cells are both from one of the following tissue types: neural tissue, epidermal tissue, dermal tissue, adipose tissue, cardiac tissue, kidney tissue, muscle tissue, liver tissue, cartilage tissue, pancreas tissue, tissue of the endometrium of uterus, umbilical cord tissue, or dental pulp tissue.