发明名称 |
Expanding cells in a bioreactor |
摘要 |
Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells. |
申请公布号 |
US9617506(B2) |
申请公布日期 |
2017.04.11 |
申请号 |
US201414542276 |
申请日期 |
2014.11.14 |
申请人 |
Terumo BCT, Inc. |
发明人 |
Jones Mark E.;Frank Nathan D. |
分类号 |
C12N1/00;C12M1/00;C12M1/12;C12M3/04;C12M1/36;C12N5/0775 |
主分类号 |
C12N1/00 |
代理机构 |
|
代理人 |
Pereyra René A.;Merkling John R.;Reagan Elizabeth J. |
主权项 |
1. A method of loading cells in a cell expansion system, the method comprising:
circulating fluid at a first flow rate through a bioreactor of a cell expansion system, wherein the fluid comprises a plurality of cells; reducing circulation of fluid from the first flow rate to about 0 ml/min; maintaining the bioreactor in a first orientation for a first predetermined period of time to allow at least a first portion of the plurality of cells to settle; after the first predetermined period of time, circulating fluid at a second flow rate through the bioreactor, wherein the second flow rate is slower than the first flow rate; reducing the circulation of fluid from the second flow rate to about 0 ml/min; maintaining the bioreactor in a second orientation for a second predetermined period of time to allow at least a second portion of the plurality of cells to settle; and expanding at least the first and the second portion of cells in the bioreactor. |
地址 |
Lakewood CO US |