Genetically engineered yeast cell producing lactate including acetaldehyde dehydrogenase, method of producing yeast cell, and method of producing lactate using the same

摘要

Provided is a genetically engineered yeast cell with lactate production capacity, including an enzyme that catalyzes conversion of acetaldehyde to acetyl-CoA and an enzyme that catalyzes conversion of pyruvate to lactate, which activities are increased compared to a parent cell of the yeast cell, as well as a method of producing the genetically engineered yeast cell and method of producing lactate using the genetically engineered yeast cell.

主权项

1. A genetically engineered yeast cell comprising,
an acylating acetaldehyde dehydrogenase enzyme of class EC 1.2.1.10 that catalyzes conversion of acetaldehyde to acetyl-CoA, and a lactate dehydrogenase enzyme of class 1.1.2.27 or EC 1.1.1.28 that catalyzes conversion of pyruvate to lactate, wherein the activity of converting acetaldehyde to acetyl-CoA and converting pyruvate to lactate is increased in the genetically engineered yeast cell as compared to a parent cell of the genetically engineered yeast cell, and the genetically engineered yeast cell produces lactate, wherein the yeast cell is of the species Saccharomyces cerevisiae.