| 发明名称 | Assay methods | ||
| 摘要 | The present disclosure is directed to a kit for the detection of an analyte of interest in a sample comprising, in one or more vials, containers, or compartments, a. a surface comprising (i) a capture reagent for the analyte, said capture reagent bound to the surface, and (ii) an anchoring reagent bound to an anchoring oligonucleotide sequence, said anchoring reagent bound to the surface, b. a first detection reagent for the analyte that is linked to a first nucleic acid probe, wherein the first nucleic acid probe comprises an extended sequence that is complementary to the anchoring oligonucleotide sequence and c. a second detection reagent for the analyte that is linked to a second nucleic acid probe. | ||
| 申请公布号 | US9618510(B2) | 申请公布日期 | 2017.04.11 |
| 申请号 | US201414206284 | 申请日期 | 2014.03.12 |
| 申请人 | MESO SCALE TECHNOLOGIES, LLC. | 发明人 | Aghvanyan Anahit;Glezer Eli N.;Kenten John;Sigal George;Stengelin Martin |
| 分类号 | G01N33/569;G01N33/533;G01N33/543;G01N33/53;C12Q1/68 | 主分类号 | G01N33/569 |
| 代理机构 | Scully, Scott, Murphy & Presser, P.C. | 代理人 | Scully, Scott, Murphy & Presser, P.C. |
| 主权项 | 1. A kit for a sandwich binding assay detection of an analyte of interest in a sample comprising, in one or more vials, containers, or compartments: a. a surface comprising (i) a capture reagent for the analyte, said capture reagent bound to the surface, and (ii) an anchoring reagent bound to an anchoring oligonucleotide sequence, said anchoring reagent bound to the surface; b. a first detection reagent for the analyte that is linked to a first nucleic acid probe, wherein the first nucleic acid probe comprises an extended sequence that is complementary to the anchoring oligonucleotide sequence bound to the anchoring reagent; and c. a second detection reagent for the analyte that is linked to a second nucleic acid probe; wherein the kit is combined to form a proximity-based detection system. | ||
| 地址 | Rockville MD US | ||