Glucosyl stevia composition

摘要

Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

主权项

1. A process for producing a highly purified glucosyl stevia composition, comprising the steps of:
adding starch into water to form a starch suspension; adding a mixture of α-amylase and CGTase into the starch suspension and incubating for about 0.5 to 2 hours at about 75-80° C., resulting in a liquefied starch suspension; inactivating the α-amylase by low pH heat treatment; cooling the liquefied starch suspension and adjusting the pH to about 5.5 to 7.0; adding steviol glycosides into the liquefied starch suspension, resulting in a reaction mixture; adding a second batch of CGTase into the reaction mixture and incubating for about 12 to 48 hours at about 55-75° C.; adding a second batch of α-amylase into the reaction mixture and incubating for a further about 12-16 hours at about 55-95° C.; inactivating the second batch of α-amylase in the reaction mixture by low pH heat treatment; adjusting the pH of the reaction mixture to about 5.5 to 7.0; decolorizing the reaction mixture; removing impurities by contacting the decolorized reaction mixture with macroporous adsorbent resin and subsequently eluting adsorbed diterpene glycosides with aqueous ethanol to result in a glycoside-containing aqueous ethanol eluate; desalting the glycoside-containing aqueous ethanol eluate with ion-exchange resins; removing ethanol from the aqueous ethanol eluate, resulting in an aqueous eluate; and concentrating and drying the aqueous eluate to obtain the highly purified glucosyl stevia composition,wherein the highly purified glucosyl stevia composition comprises α-1,4-glucosyl derivatives, and unmodified steviol glycosides.