Fabrication method for stratified and layered tissue to repair osteochondral defects

摘要

Fabrication method for stratified and layered tissue to repair osteochondral defects. In a method of the present disclosure, the method comprises the step of applying a first direction magnetic field to a first quantity of a first collagen solution to align collagen within the first collagen solution in a first direction relative to the first direction magnetic field, forming a first layer of collagen. In a method of generating an aligned collagen layer of the present disclosure, the method comprises applying a first magnetic field at or greater than 0.1 Tesla to a layer of a first collagen solution defining a horizontal plane, within a temperature at or between 2° C. and 45° C., to generate an aligned collagen layer.

主权项

1. A method of generating an aligned collagen layer, comprising:
applying a first magnetic field at or above 0.1 Tesla to a layer of a first collagen solution defining a horizontal plane, within a temperature at or between 2° C. and 45° C., to align a plurality of collagen fibrils formed within the first collagen solution before fibrillogenesis is complete and generate an aligned collagen layer having a planar fibril alignment and a thickness; selecting or altering the temperature as a tunable parameter to manipulate the rate of fibrillogenesis of the collagen fibrils to achieve a targeted degree of alignment of the collagen fibrils across the entirety of the thickness of the aligned collagen layer; combining the aligned collagen layer with a second layer of collagen, the second layer of collagen positioned adjacent to the aligned collagen layer to form a construct and comprising a quantity of collagen solution selected from a group consisting of a first quantity of a second collagen solution and a second quantity of the first collagen solution; and adding to the construct by combining the aligned collagen layer and the second layer of collagen with a third layer of collagen, the third layer of collagen positioned adjacent to one or both of the aligned collagen layer and the second layer of collagen and comprising a quantity of collagen solution selected from a group consisting of the first quantity of the second collagen solution, a second quantity of the second collagen solution, the second quantity of the first collagen solution, and a third quantity of the first collagen solution; wherein the temperature is tuned such that the step of applying takes longer than 30 minutes to achieve at least 95% fibrillogenesis of the collagen fibrils and at least one of the second and third layers of collagen of the construct consists of collagen fibrils that are randomly aligned.