SiRNA hydrogel and method for manufacturing the same

摘要

The present invention relates to siRNA hydrogel and a method for manufacturing the same, and more particularly, to siRNA hydrogel for targeted gene silencing, which is nano-structured for targeted gene silencing, and a method for manufacturing the same.

主权项

1. An siRNA hydrogel having a three dimensional network structure comprising pluralities of sense conjugate 1and antisense conjugate 1sense conjugate 1and antisense conjugate 2or antisense conjugate 1and sense conjugate 2 wherein (a) X is a sense strand of siRNA, (b) Y is an antisense strand of siRNA,is tris-(2-maleimidoethyl)-amine (TMEA),
(d) “•” is a bond formed between X or Y andis a linkage mediated by dithio-bis-maleimidoethane(DTME) or 1,8-bis-maleimidodiethyleneglycol BM(PEG)2 between the Ys in antisense conjugate 2 or the Xs in sense conjugate 2,
(f) • andare formed through the reaction of a functional group introduced to an end of X and Y prior to formation of • andwherein each of the functional groups may be the same or different, and
(g) the three dimensional network structure comprises crosslinking between the plurality of sense and antisense conjugates through complementary hybridization between X and Y in adjacent sense and antisense conjugates.