METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF FATTY ALDEHYDES AND FATTY ALCOHOLS

摘要

The invention relates to the use of EntD polypeptides, polynucleotides encoding the same, and homologues thereof to enhance the production of fatty aldehydes and fatty alcohols in a host cell.

主权项

1. A method of producing a fatty aldehyde or a fatty alcohol in a host cell, comprising:
(a) expressing a polynucleotide sequence encoding a phosphopanthetheinyl transferase (PPTase) comprising an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 1 in the host cell, (b) culturing the host cell expressing the PPTase in a culture medium under conditions permissive for the production of a fatty aldehyde or a fatty alcohol, and (c) isolating the fatty aldehyde or fatty alcohol from the host cell, with the proviso that if the polynucleotide sequence encodes an endogenous PPTase, then the endogenous PPTase is overexpressed.