Novel Ecdysone Receptor/Invertebrate Retinoid X Receptor-Based Inducible Gene Expression System

摘要

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel ecdysone receptor/invertebrate retinoid X receptor-based inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large-scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

主权项

1. A gene expression modulation system comprising:
a) a first gene expression cassette that is capable of being expressed in a host cell comprising a polynucleotide that encodes a first hybrid polypeptide comprising:
i) a DNA-binding domain that recognizes a response element associated with a gene whose expression is to be modulated; andii) an ecdysone receptor ligand binding domain; and b) a second gene expression cassette that is capable of being expressed in the host cell comprising a polynucleotide that encodes a second hybrid polypeptide comprising:
i) a transactivation domain; and ii) an invertebrate retinoid X receptor ligand binding domain.