DEVELOPMENT AND USE OF FLUORESCENT PROBES OF UNBOUND BILIRUBIN

摘要

Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.

主权项

1. A probe comprising an (iLBP), wherein the iLBP corresponds to SEQ ID NO: 3 comprising one or more amino acid substitutions and a fluorophore wherein the fluorophore is attached to a lysine residue, the N-terminus amino group of the iLBP, or to a cysteine substitution, and wherein the probe binds to bilirubin but does not significantly bind to fatty acid.