| 摘要 |
At least first and second digital images of the sample are acquired having different focal heights relative to a platform on which the cells are disposed. A contrast matrix is produced having elements computed in dependence upon the difference between the values of the corresponding pixels in the first and second images. A phase matrix is produced by convolution of the contrast matrix with a predetermined distance matrix. The phase matrix is used to assess characteristics of the sample, such as the presence of cells in the sample or the heights of cells in the sample. The application also discloses use of a flashing light source to illuminate the sample, measuring the height of the platform based on the image of a beam reflected into the objective element of the imaging system and illuminating the sample with different colours of light and combining the images so produced. |
