CULTURE MEDIUM AND CULTURING METHOD FOR ANCHORAGE-DEPENDENT CELLS, CELL COMPOSITION INCLUDING STEM CELLS AND/OR DIFFERENTIATED CELLS DERIVED FROM STEM CELLS, AND PRODUCTION METHOD FOR CELL COMPOSITION

摘要

As a technique capable of culturing anchorage-dependent cells without using an anchorage, provided is a medium for anchorage-dependent cells, which comprises MFG-E8 (Milk fat globule-EGF factor 8) or a fragment of the protein. This medium can promote adhesion of anchorage-dependent cells in the absence of an anchorage, enables the survival and proliferation (colony formation) of the cells, and further, also enables the subsequent differentiation if the anchorage-dependent cells are stem cells.