Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in cells and tissue

摘要

The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.

主权项

1. A method for enhancing the signal-to-noise ratio from an emission comprising:
selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more high repetition energy pulses, wherein the pulses are timed to allow time for excited states of molecules in the probe to depopulate complete after a series of pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.