Complex miRNA sets as novel biomarkers for an acute coronary syndrome

摘要

The present invention relates to single polynucleotides or sets of polynucleotides for detecting single miRNAs or sets of miRNAs for diagnosing and/or prognosing of an acute coronary syndrome in a blood sample from a human. Further, the present invention relates to means for diagnosing and/or prognosing of an acute coronary syndrome comprising said polynucleotides or sets of polynucleotides. Furthermore, the present invention relates to a method for diagnosing and/or prognosing of an acute coronary syndrome based on the determination of expression profiles of single miRNAs or sets of miRNAs representative for an acute coronary syndrome compared to a reference. In addition, the present invention relates to a kit for diagnosing and/or prognosing of an acute coronary syndrome comprising means for determining expression profiles of single miRNAs or sets of miRNAs representative for an acute coronary syndrome and at least one reference.

主权项

1. A method for detecting a decreased level of at least one miRNA in a human patient having, or suspected of having, an acute coronary syndrome comprising the steps of:
(i) providing a blood cell sample from a human, wherein the blood cell sample is a blood cellular fraction consisting of a mixture of erythrocytes, leukocytes and thrombocytes, and (ii) detecting whether there is a decreased level of the at least one miRNA in the blood cell sample as compared to a control level of a healthy human subject, or a human subject not having or not being suspected of having an acute coronary syndrome, by contacting the sample with at least one probe for at least one down-regulated miRNA and detecting binding between the probe and the down-regulated miRNA; wherein the nucleotide sequence of said at least one down-regulated miRNA is miR-455-3p having SEQ ID NO: 16, or sequence having at least 95% sequence identity thereto.