Endoglucanase 1B (EG1B) variants

摘要

The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.

主权项

1. A variant endoglucanase or enzymatically active fragment thereof comprising
(a) an amino acid sequence at least 80% identical to the wild-type endoglucanase amino acid sequence of SEQ ID NO:3; and (b) an amino acid substitution introducing an amino acid other than phenylalanine at position 63, wherein the residue positions are numbered with reference to SEQ ID NO:2.