Sensor chip, detection method, and detection apparatus

摘要

An exemplary sensor chip includes a substrate, a metal pattern, a proximate substance, and a light shielding layer. The metal pattern is on the substrate. The proximate substance is on or near the metal pattern. The light shielding layer is provided on the substrate so as to cover the metal pattern and the proximate substance. The light shielding layer is a layer that blocks the excitation light from going into the proximate substance, and is made of a substance which becomes degraded inside a subject.

主权项

1. A sensor chip for detecting an analyte inside a subject, comprising
a substrate, a metal pattern, a proximate substance, and a light shielding layer, the metal pattern being on one face of the substrate, and the proximate substance being on or near the metal pattern, wherein, the metal pattern induces a surface plasmon wave by being irradiated with excitation light; the proximate substance generates first surface-enhanced Raman-scattered light by being irradiated with the excitation light, the first surface-enhanced Raman-scattered light having a first peak; the first surface-enhanced Raman-scattered light does not vary with changes in the concentration of the analyte; the proximate substance is a substance which binds to the analyte; the proximate substance having bound to the analyte generates second surface-enhanced Raman-scattered light by being irradiated with the excitation light, the second surface-enhanced Raman-scattered light having a second peak different from the first peak; the second surface-enhanced Raman-scattered light varies with changes in the concentration of the analyte; the light shielding layer is provided on the substrate so as to cover the metal pattern and the proximate substance; the light shielding layer is a layer which blocks the excitation light from going into the proximate substance; and the light shielding layer is made of a substance which becomes degraded inside the subject.