METHOD OF PRODUCING IMMUNOGLOBULIN FC REGION INCLUDING INITIAL METHIONINE RESIDUE

摘要

The present invention relates to a method of producing a monomeric or dimeric immunoglobulin Fc region including the initial methionine residue using a vector including a nucleotide sequence encoding a recombinant immunoglobulin Fc region including an immunoglobulin hinge region, and to a monomeric or dimeric immunoglobulin Fc region including the initial methionine residue prepared by the method. A recombinant immunoglobulin Fc region including an immunoglobulin hinge region of the present invention may be used to produce a recombinant immunoglobulin Fc region as inclusion bodies in E. coli, and methionine is included in the expressed recombinant immunoglobulin Fc region. The produced immunoglobulin Fc region is linked to a physiologically active polypeptide, and is thereby used for increasing serum half-life of the physiologically active polypeptide.