MICROSCOPE DEVICE AND IMAGE ACQUISITION METHOD

摘要

A microscope apparatus includes an objective lens; a light source for outputting light with which a biological sample is irradiated via the objective lens; a shape acquisition unit for acquiring information on at least one of a surface shape of the biological sample and a structure directly under the surface of the biological sample; a hologram generation unit for generating aberration correction hologram data for correcting an aberration caused by the at least one on the basis of the information acquired by the shape acquisition unit; a spatial light modulator to which a hologram based on the aberration correction hologram data is presented and for modulating the light output from the light source; a photodetector for detecting an intensity of light generated in the biological sample and outputs a detection signal.

主权项

1: A microscope apparatus for acquiring an image of a biological sample, the microscope apparatus comprising:
a biological sample table configured to support the biological sample; an objective lens disposed to face the biological sample table; a light source configured to output light with which the biological sample is irradiated via the objective lens; a shape acquisition unit configured to acquire information on at least one of a surface shape of the biological sample and a structure directly under the surface of the biological sample; a hologram generation unit configured to generate aberration correction hologram data for correcting an aberration caused by the at least one on the basis of the acquired information; a first spatial light modulator configured to modulate the light output from the light source based on the aberration correction hologram data; a photodetector configured to detect an intensity of light generated in the biological sample and outputting a detection signal; and an image generation unit configured to generate an image of the biological sample on the basis of the detection signal.