| 发明名称 |
MUTANT ENZYME AND PRODUCTION METHOD FOR TERPENOID USING SAID MUTANT ENZYME |
| 摘要 |
The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising:(a) an amino acid other than alanine (A) at the 10th position in an Sα2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an Lα2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an Lα2 amino acid sequence of HMGR of the present invention. |
| 申请公布号 |
US2017088824(A1) |
申请公布日期 |
2017.03.30 |
| 申请号 |
US201515302903 |
申请日期 |
2015.04.09 |
| 申请人 |
ADEKA CORPORATION |
发明人 |
TAKEHANA Toshihiko;KOIKE Seiji;KUZUYAMA Tomohisa |
| 分类号 |
C12N9/04;C12N15/52;C12P5/00 |
主分类号 |
C12N9/04 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A hydroxymethylglutaryl CoA reductase (HMGR) comprising:
(a) an amino acid other than alanine (A) at the 10th position in an Sα2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an Lα2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an Lα2 amino acid sequence of HMGR. |
| 地址 |
Tokyo JP |
