METHODS OF ANALYTE DERIVATIZATION AND ENHANCED SOFT IONIZATION

摘要

Methods of analyte derivatization and soft ionization are provided. The methods include contacting a sample including an analyte with a derivatization agent to produce a modified analyte including a pseudo-molecular analyte group and a leaving group connected via a fragmentable bond; and selectively breaking the fragmentable bond under soft ionization conditions to produce a predominant first fragmentation product including the pseudo-molecular analyte group and a second fragmentation product including the leaving group. The method may further include analyzing the first and second fragmentation products in a mass spectrometer to identify an ion corresponding to the pseudo-molecular analyte group. Also provided are methods for detecting analytes using gas chromatography-mass spectroscopy (GC-MS). These methods find use in a variety of applications in which mass spectroscopic analysis of samples is desired.

主权项

1. A method comprising:
(a) contacting a sample comprising an analyte with a derivatization agent to produce a modified analyte comprising a pseudo-molecular analyte group and a leaving group connected via a fragmentable bond; and (b) selectively breaking the fragmentable bond under soft ionization conditions, thereby producing a predominant first fragmentation product comprising the pseudo-molecular analyte group and a second fragmentation product comprising the leaving group.