MICROFLUIDIC CAPTURE AND DETECTION OF BIOLOGICAL ANALYTES

摘要

A microfluidic capture device is described that include a regular macroporous layer comprising a binding molecule, an inlet and outlet port fluidly connected to the regular macroporous layer and configured to add or remove a biological sample to the regular macroporous layer and positioned to allow flow of the biological sample through a flow region in the regular macroporous layer, a first and second electrode positioned on opposite sides of the flow region, and a substrate enclosing the regular macroporous layer, including top and bottom sides on opposite sides of the regular macroporous layer. Methods of using the device to qualitatively and/or quantitatively determine the amount of a biological analyte such as a virus particle in a biological sample obtained from a subject using cyclic voltammetry are also described.

主权项

1. A microfluidic capture device, comprising:
a regular macroporous layer comprising a binding molecule, an inlet and outlet port fluidly connected to the regular macroporous layer and configured to add or remove a biological sample to the regular macroporous layer and positioned to allow flow of the biological sample through a flow region in the regular macroporous layer, a first and second electrode positioned on opposite sides of the flow region, and a substrate enclosing the regular macroporous layer, including top and bottom sides on opposite sides of the regular macroporous layer.