发明名称 |
METHOD FOR INDUCING DIFFERENTIATION OF INSULIN-PRODUCING CELLS |
摘要 |
It is an object of the present invention to provide a method for efficiently directing differentiation into insulin-producing cells in a xeno-free culture system. According to the present invention, there is provided a method for directed differentiation into insulin-producing cells, comprising culturing stem cells in the following steps (1) to (5): (1) a step of culturing stem cells in a medium comprising an activator of activin receptor-like kinase-4/-7 and a GSK3 inhibitor and then culturing in a medium comprising an activator of activin receptor-like kinase-4/-7; (2) a step of culturing the cells obtained in step (1) in a medium comprising a hedgehog signaling inhibitor and an FGF; (3) a step of culturing the cells obtained in step (2) in a medium comprising a retinoic acid receptor agonist, a hedgehog signaling inhibitor and a BMP signaling inhibitor; (4) a step of culturing the cells obtained in step (3) in a medium comprising a TGF-² type I activin receptor-like kinase-4/-5/-7 inhibitor and a BMP signaling inhibitor; and (5) a step of culturing the cells obtained in step (4) in a medium comprising a phosphodiesterase inhibitor. |
申请公布号 |
EP3147356(A1) |
申请公布日期 |
2017.03.29 |
申请号 |
EP20150795718 |
申请日期 |
2015.05.19 |
申请人 |
Tokyo Institute of Technology |
发明人 |
KUME, Shoen;SHIRAKI, Nobuaki |
分类号 |
C12N5/0735;A61K35/12;A61P3/10;C12N5/074;C12N5/10;C12N15/09;C12Q1/02;G01N33/15;G01N33/50 |
主分类号 |
C12N5/0735 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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