发明名称 |
DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR |
摘要 |
Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product. |
申请公布号 |
EP2456855(B1) |
申请公布日期 |
2017.03.29 |
申请号 |
EP20100802935 |
申请日期 |
2010.07.23 |
申请人 |
GE Healthcare Bio-Sciences Corp. |
发明人 |
GUERTIN, Patrick, M.;CROWELL, Joseph, D. |
分类号 |
C12N7/00;C12M1/00;C12M1/12 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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