| 主权项 |
1. A method of producing a sugar solution, comprising (1) to (3):
(1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulase; (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry; and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate, wherein the inactivated cellulase in (1) is prepared by dipping the hydrolysis residue in (3) in an alkaline aqueous solution selected from a group consisting of ammonia, sodium hydroxide, potassium hydroxide, calcium hydroxide, calcium carbonate, sodium carbonate, and trisodium phosphate, having a pH 11 or more and a temperature of less than 65° C., and wherein the inactivated cellulase is used in (1) of the next and later production process. |
