PREPARATION OF SODIUM HYALURONATE USING A MUTANT OF STREPTOCOCCUS ZOOEPIDEMICUS

摘要

Disclosed is a process for the production of sodium hyaluronate with a molecular weight of between 60 and 2400 kDa and low polydispersity (1.4 Mw/Mn), which comprises: a) a step of fermentation of Streptococcus equi subsp. Zooepidemicus CNCM 1-4645 in a suitable culture medium; b) a step of ultrafiltration of the cell-free filtered solution; by concentrating and diafiltering the solution under differential pressure conditions (&Dgr;P) of 1.0-5.0 bar(g) and transmembrane pressure (TMP) of 0.5-4 bar(g).