主权项 |
1. A method of detecting solid tumor stem cells, wherein the method comprises:
(i) obtaining cells from a tumor of a subject; (ii) detecting cells that are positive for:
(a) at least one protein selected from the group consisting of CD44, CD24, ESA, ALDH, TPRXL, NOTCH2, RBM15, ST3GAL3, NFYA, PCNX, TAS2R14, CD300LB, GIPC3, RAD51L1, ARID1B, EPPK1, COL11A2; KLK3, EIF2C2; ZFP41, FAM49B, and PSORS1C2 among said cells from the tumor, or(b) ALDH activity among said cells from the tumor; and (iii) detecting cells that are positive for CXCR1 or FBXO21 among said cells from the tumor, wherein the detecting step for cells positive for CXCR1 comprises the step of contacting said cells with Compound 1: and wherein the presence of cells positive for CXCR1 or FBXO21 and cells positive for at least one protein selected from the group consisting of CD44, CD24, ESA, ALDH, TPRXL, NOTCH2, RBM15, ST3GAL3, NFYA, PCNX, TAS2R14, CD300LB, GIPC3, RAD51L1, ARID1B, EPPK1, COL11A2; KLK3, EIF2C2; ZFP41, FAM49B, and PSORS1C2 or ALDH activity indicate the presence of solid tumor stem cells in said tumor. |