发明名称 |
Method for extracting IgY (γ-livetin) from egg yolk |
摘要 |
A method for extracting IgY (γ-livetin) from yolk is disclosed, which comprises the following steps: (A) providing a buffer solution, a yolk sample, and an inorganic salt solution; (B) diluting the yolk sample with the buffer solution to obtain a mixture, stirring the mixture for a predetermined time, and performing a centrifugation on the mixture to obtain a supernatant; and (C) adding the inorganic salt solution into the supernatant to salt out IgY, wherein a pH value of the buffer solution is in a range from 4.6 to 5.4, a salt concentration of the buffer solution is in a range from 0.05 M to 0.15 M, and a saturation degree of the inorganic salt solution is in a range from 30% to 60%. |
申请公布号 |
US9605052(B2) |
申请公布日期 |
2017.03.28 |
申请号 |
US201313956511 |
申请日期 |
2013.08.01 |
申请人 |
NATIONAL TAIWAN NORMAL UNIVERSITY |
发明人 |
Wang Yu-Chie;Wang Zong-Ming |
分类号 |
C07K1/14;C07K1/30;C07K16/02 |
主分类号 |
C07K1/14 |
代理机构 |
Bacon & Thomas, PLLC |
代理人 |
Bacon & Thomas, PLLC |
主权项 |
1. A method for extracting γ-livetin (IgY) from yolk, consisting of the following steps:
(A) providing a buffer solution, a yolk sample, and an inorganic salt solution; (B) diluting the yolk sample with the buffer solution to obtain a mixture, stirring the mixture for a predetermined time, and performing a centrifugation on the mixture to obtain a supernatant; and (C) adding the inorganic salt solution into the supernatant to salt out IgY, wherein a pH value of the buffer solution is in a range from 4.6 to 5.4, a salt concentration of the buffer solution is in a range from 0.05 M to 0.15 M, the predetermined time for stirring the mixture is 25 min or more, and a saturation degree of the inorganic salt solution is in a range from 30% to 60%. |
地址 |
TW |