发明名称 |
Oligonucleotide-based probes for detection of bacterial nucleases |
摘要 |
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base. |
申请公布号 |
US9603949(B2) |
申请公布日期 |
2017.03.28 |
申请号 |
US201214342338 |
申请日期 |
2012.08.30 |
申请人 |
University of Iowa Research Foundation;Integrated DNA Technologies, Inc. |
发明人 |
McNamara, II James O.;Stockdale Katie R.;Huang Lingyan;Horswill Alexander R.;Behlke Mark A.;Hernandez Frank J. |
分类号 |
C12Q1/68;C07H21/00;C12P19/34;A61K49/00;G01N33/569;C12Q1/44;G01N33/542 |
主分类号 |
C12Q1/68 |
代理机构 |
Viksnins Harris & Padys PLLP |
代理人 |
Viksnins Harris & Padys PLLP |
主权项 |
1. A probe for detecting a microbial endonuclease comprising an oligonucleotide of 2-30 nucleotides in length, a fluorophore operably linked to the oligonucleotide, and a quencher operably linked to the oligonucleotide, wherein the oligonucleotide comprises a chemically modified pyrimidine comprising a 2′ substituted sugar and is capable of being cleaved by a microbial nuclease but not by a mammalian nuclease. |
地址 |
Iowa City IA US |