发明名称 |
Materials presenting notch signaling molecules to control cell behavior |
摘要 |
The invention provides a solution to the problem of delivering molecules in a physiologically relevant manner to direct cell fate. For example, a Notch ligand molecule is presented in a microenvironment that mimics the environment encountered in vivo. Accordingly, the invention features a cell delivery vehicle comprising a biocompatible hydrogel or polymer and a composition that binds to a Notch receptor and methods of directing cell fate, e.g., stem cell differentiation, using such compositions. |
申请公布号 |
US9603894(B2) |
申请公布日期 |
2017.03.28 |
申请号 |
US201113883005 |
申请日期 |
2011.11.08 |
申请人 |
President and Fellows of Harvard College |
发明人 |
Cao Lan;Bouhadir Kamal H.;Mooney David J. |
分类号 |
A61K38/10;A61K38/17;A61K47/48 |
主分类号 |
A61K38/10 |
代理机构 |
McCarter & English, LLP |
代理人 |
McCarter & English, LLP ;Zacharakis Maria Laccotripe |
主权项 |
1. A method of inducing differentiation of a population of mesenchymal stem cells comprising transiently contacting the population of mesenchymal stem cells with:
a) a biocompatible alginate hydrogel covalently linked to human Jagged-1, said Jagged-1 comprising the amino acid sequence of SEQ ID NO:1, or a fragment thereof, said fragment comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:3, and b) a bone morphogenetic protein, thereby inducing differentiation of the population of mesenchymal stem cells into a population of cells of osteogenic lineage, wherein the level of alkaline phosphatase activity of the population of cells after said contacting is increased by at least 4-fold as compared to the level of alkaline phosphatase activity of a population of mesenchymal stem cells that has been contacted with the bone morphogenetic protein but not the alginate hydrogel. |
地址 |
Cambridge MS US |