Compositions and methods for restoring gastrointestinal microbiota following antibiotic treatment

摘要

The present invention relates to characterizing changes in mammalian bacterial gastrointestinal, cutaneous and nasal microbiota associated with antibiotic treatment and various disease conditions (such as asthma, allergy, obesity, metabolic syndrome, gastrointestinal reflux disease (GERD), eosinophilic esophagitis, gastro-esophageal junction adenocarcinomas (GEJAC), infections due to bacteria that are resistant to antibiotics, including Methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile, vancomycin-resistant enterococci, etc.) and related diagnostic and therapeutic methods. Therapeutic methods of the invention involve the use of live bacterial inoculants that are capable of restoring healthy mammalian bacterial gastrointestinal, skin, and nasal microbiota.

主权项

1. A method for treating antibiotic-induced obesity by restoring one or more bacterial species depleted in a gastrointestinal microbiota of a mammal following an antibiotic treatment, wherein said mammal has developed or is at risk of developing obesity as a result of the depletion of said bacterial species in the gastrointestinal microbiota of said mammal, said method comprising:
a) identifying one or more bacterial species which is statistically significantly diminished in a gastrointestinal microbiota sample obtained from the mammal following the antibiotic treatment as compared to a control gastrointestinal microbiota; b) culturing bacteria from the one or more bacterial species determined in step (a) to be statistically significantly diminished in said sample as compared to the control; and c) administering the cultured bacteria from step (b) to the mammal in an amount sufficient to increase the abundance of said bacteria in the gastrointestinal microbiota of said mammal and treat said antibiotic-induced obesity in said mammal.