Enhancing spatial resolution utilizing multibeam confocal scanning systems

摘要

A method for utilizing a multi-beam confocal scanning system to generate an image of a sample, the image having an improved resolution, is provided. An array of beams may be positioned at a first location on the sample. A first plurality of images may be captured, where each of the first plurality of images is associated with a beam of the array of beams at the first location. The array of beams may be adjusted by a specific distance to a second location on the sample, the specific distance being smaller than an optical resolution limit of the multi-beam confocal scanning system. A second plurality of images may be captured, where each of the second plurality of images is associated with a beam of the array of beams at the second location.

主权项

1. A method for utilizing a multi-beam confocal scanning system to generate an image of a sample, the image having an improved resolution, the method comprising:
scanning the sample utilizing an array of beams of the multi-beam confocal scanning system, the scanning comprising the array of beams appearing stationary at multiple locations on the sample; capturing a plurality of secondary images at each location of the multiple locations on the sample; dividing each of the plurality of secondary images into an array of pixel intensities; and collecting the array of pixel intensities to be processed to create the image having the improved resolution.