| 主权项 |
1. An assay kit for concurrently detecting H. influenzae, M. catarrhalis and S. pneumoniae from a mucosal sample, the assay kit comprising:
a lysis buffer to lyse cells within the sample and form a single sample solution, wherein the lysis buffer comprises between 0.01% and 5% (w/w) of an anionic surfactant and between 0.1% and 15% (w/w) of an osmotic agent, wherein the anionic surfactant of the lysis buffer comprises sarkosyl and wherein the osmotic agent of the lysis buffer comprises sucrose; a cartridge containing one or more solid phase substrates holding a first agent that binds specifically to a first antigen specific to H. influenzae but not M. catarrhalis or S. pneumoniae, a second agent that binds specifically to a second antigen specific to M. catarrhalis but not H. influenzae or S. pneumoniae, and a third agent that binds specifically to a third antigen specific to S. pneumoniaebut not M. catarrhalis or H. influenzae, wherein the first, second and third agents are bound to specific regions of the one or more solid phase substrates in the cartridge; p1 one or more conjugation regions within the cartridge, the one or more conjugation regions in fluid communication with the one or more solid phase substrates and comprising a fourth agent that is labeled and that binds specifically to the first antigen, a fifth agent that is labeled and that binds specifically to the second antigen, and a sixth agent that is labeled and that bind specifically to the third antigen; one or more sample inlets on the cartridge in fluid communication with the one or more conjugation regions; and one or more windows through which the specific regions of the solid phase substrate to which the first, second and third agents are bound may be viewed. |
